Developmental Toxicity Testing of E-Cigarettes Using Drosophila melanogaster Embryonic Stem Cells
Abstract
E-cigarettes (EC) are relatively new and many of the effects they have on the body are still unknown. Given this, even less is known about the effects they have on embryonic development. Previously identified as a reliable method for teratogen testing, Drosophila melanogaster embryos fully develop and hatch into larvae 24 hours after oviposition which makes them attractive as a fast and reliable method of testing developmental toxicants (Bournias-Vardiabasis, 1983). When primary cultures are taken at the gastrula stage of development, the embryonic stem cells will differentiate into the beginnings of the muscular and neuronal systems in vitro within 24 hours and these differentiated cells called myotubes and neuronal clusters can be counted. In this study, primary cultures of D. melanogaster embryos at the gastrula stage
Developmental Toxicity Testing of E-Cigarettes Using Drosophila melanogaster Embryonic Stem Cells
Event Center A & B
E-cigarettes (EC) are relatively new and many of the effects they have on the body are still unknown. Given this, even less is known about the effects they have on embryonic development. Previously identified as a reliable method for teratogen testing, Drosophila melanogaster embryos fully develop and hatch into larvae 24 hours after oviposition which makes them attractive as a fast and reliable method of testing developmental toxicants (Bournias-Vardiabasis, 1983). When primary cultures are taken at the gastrula stage of development, the embryonic stem cells will differentiate into the beginnings of the muscular and neuronal systems in vitro within 24 hours and these differentiated cells called myotubes and neuronal clusters can be counted. In this study, primary cultures of D. melanogaster embryos at the gastrula stage
