The author of this document has limited its availability to on-campus or logged-in CSUSB users only.
Off-campus CSUSB users: To download restricted items, please log in to our proxy server with your MyCoyote username and password.
Date of Award
Restricted Thesis: Campus only access
Master of Science in Biology
First Reader/Committee Chair
Myocardial infarctions affect approximately 735,000 people annually in the United States and have a substantial impact on the quality of life. Neonates have an enhanced capability of repairing cardiovascular damage, while adult human heart tissue does not regenerate. The mechanistic basis for this age-dependent difference in regenerative capacity remains unknown. Recent studies have shown that microRNAs (miRNAs) play a significant role in the regenerative ability of cardiac stem cells. The objective of this project is to distinguish alterations in miRNA expression in myocardial infarcted sheep hearts following injection of ovine neonatal ISL1+ cardiac progenitor cells (CPCs) for cardiac repair. We hypothesize that transcripts encoding miRNAs associated with enhanced stemness, cell division, and survival will be differentially regulated. To understand the role of miRNA expression associated with the regenerating myocardium, a sheep model of myocardial infarction was used to identify the miRNAs induced in the neonatal CPC-treated region of the myocardial repair. Nine microRNAs were differentially regulated in vivo, including miR-99, miR-100, miR-302a, miR-208a, miR-665, miR-1, miR-499a, miR-34a, and miR-133a. Each of these microRNAs function in promoting cardiovascular regeneration. Our study validated the role of select miRNAs and identified several new miRNA transcripts involved in neonatal CPC-mediated cardiovascular repair. This information can be used to design new approaches to further optimize cell-based treatments for the heart.
Lopez, Larry Valentino Jr, "DIFFERENTIALLY EXPRESSED MICRORNAS DURING NEONATAL CARDIOVASCULAR PROGENITOR CELL-MEDIATED CARDIAC REPAIR" (2020). Electronic Theses, Projects, and Dissertations. 1066.