Event Title

Toxicologic effects of the glyphosate-based herbicide Roundup® on Canton-S Drosophila melanogaster reproductive function.

Presenter Information

Kelly Muller

Presentation Type

Poster Presentation/Art Exihibt

College

College of Natural Sciences

Major

Chemistry and Biochemistry

Location

SMSU Event Center BC

Faculty Mentor

Dr. Becky Talyn

Start Date

5-17-2018 9:30 AM

End Date

5-17-2018 11:00 AM

Abstract

Glyphosate is the active ingredient in widely used herbicides, including Roundup®. Evidence shows that glyphosate, although effective, is toxic to some organisms and their endocrine systems; however, its 50 5th Annual Student Research Symposium toxicity to humans remains unclear. This study utilized a Canton-S strain of Drosophila melanogaster to explore the effects of glyphosate exposure on reproductive anatomy and function. Flies were exposed to organic medium with or without one of two homeowner grade Roundup® formulations containing glyphosate with pelargonic acid or with POEA. Each formulation was administered at glyphosate concentrations of 0.0 g/L, 0.5 g/L, 1.0 g/L, and 2.0 g/L. After 7 days of exposure, the organism’s ovaries, oocytes, and spermatheca were dissected and scored, in addition to mortality. Flies exposed to Roundup® with pelargonic acid at 2 g/L experienced decreased survival, a lower concentration than decreased survival when other Roundup® formulations were used. With repeated trials, we expect that this concentration of Roundup® with pelargonic acid will yield the highest mortality rate of Drosophila. Literature from studies about other species suggests that glyphosate will decrease Drosophila ovary size, number of oocytes, and presence of sperm in the spermatheca. If the expected results are found after the completion of 25 female Drosophila dissections per treatment, we will conclude that glyphosate based herbicide in the form of Roundup® is toxic to Drosophila melanogaster, specifically affecting reproductive anatomy, function and success.

Share

COinS
 
May 17th, 9:30 AM May 17th, 11:00 AM

Toxicologic effects of the glyphosate-based herbicide Roundup® on Canton-S Drosophila melanogaster reproductive function.

SMSU Event Center BC

Glyphosate is the active ingredient in widely used herbicides, including Roundup®. Evidence shows that glyphosate, although effective, is toxic to some organisms and their endocrine systems; however, its 50 5th Annual Student Research Symposium toxicity to humans remains unclear. This study utilized a Canton-S strain of Drosophila melanogaster to explore the effects of glyphosate exposure on reproductive anatomy and function. Flies were exposed to organic medium with or without one of two homeowner grade Roundup® formulations containing glyphosate with pelargonic acid or with POEA. Each formulation was administered at glyphosate concentrations of 0.0 g/L, 0.5 g/L, 1.0 g/L, and 2.0 g/L. After 7 days of exposure, the organism’s ovaries, oocytes, and spermatheca were dissected and scored, in addition to mortality. Flies exposed to Roundup® with pelargonic acid at 2 g/L experienced decreased survival, a lower concentration than decreased survival when other Roundup® formulations were used. With repeated trials, we expect that this concentration of Roundup® with pelargonic acid will yield the highest mortality rate of Drosophila. Literature from studies about other species suggests that glyphosate will decrease Drosophila ovary size, number of oocytes, and presence of sperm in the spermatheca. If the expected results are found after the completion of 25 female Drosophila dissections per treatment, we will conclude that glyphosate based herbicide in the form of Roundup® is toxic to Drosophila melanogaster, specifically affecting reproductive anatomy, function and success.